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The mTORC1 complex and free RAPTOR were separated with a 20-ml salt gradient to a final concentration of 0.5 M NaCl, using salt-free buffer and high-salt buffer (50 mM HEPES, 1 M NaCl, 1 mM TCEP, pH 7.4)

J Neurol 2022 Correll CU et al

While traditional medicine relies on specific regulatory types to handle treatment based on symptoms and the patient's general profile, it is also crucial to recognize cross-cultural factors in preventive and therapeutic approaches, particularly understanding shared and distinct pathological, physiological, and psychological features (44)

Combination Approaches These approaches are not mutually exclusive